Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 49, Issue 4, Pages 556-567Publisher
WILEY
DOI: 10.1111/j.1744-7909.2007.00442.x
Keywords
binary vectors; gene silencing; green fluorescent protein fusion; high-throughput; promoter analysis; recombination-assisted cloning
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A series of high-throughput binary cloning vectors were constructed to facilitate gene function analysis in higher plants. This vector series consists of plasmids designed for plant expression, promoter analysis, gene silencing, and green fluorescent protein fusions for protein localization. These vectors provide for high-throughput and efficient cloning utilizing sites for lambda phage integrase/excisionase. In addition, unique restriction sites are incorporated in a multiple cloning site and enable promoter replacement. The entire vector series are available with complete sequence information and detailed annotations and are freely distributed to the scientific community for non-commercial uses.
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