Cryobacterium psychrotolerans sp nov, a novel psychrotolerant bacterium isolated from the China No 1 glacier

A novel psychrotolerant, Gram-positive, yellow-pigmented, aerobic bacterium, strain 0549(T), was isolated from the China No. 1 glacier. The cells of the isolate were catalase-positive, motile, irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain 0549(T) was able to grow at 4-27 degrees C, with optimum growth occurring at 20-22 degrees C. The major fatty acids were anteiso-C-15:0, anteiso-C-15:1, iso-C-16:0 and anteiso-C-17:0. The genomic; DNA G + C content was 67 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 0549(T) belonged to the genus Cryobacterium T and exhibited 96.5 % similarity to Cryobacterium psychrophilum, JCM 1463. On the basis of the phenotypic, chemotaxonomic and phylogenetic properties and the DNA-DNA relatedness data, strain 0549(T) represents a novel species of the genus Cryobacterium, for which the name Cryobacterium, psychrotolerans is proposed. The type strain is 0549(T) (=CGMCC 1.5382(T)=JCM 13925(T)).