Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 73, Issue 7, Pages 2324-2328Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02038-06
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Funding
- NCI NIH HHS [CA112418, R21 CA112418] Funding Source: Medline
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An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.
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