Journal
BIORESOURCE TECHNOLOGY
Volume 98, Issue 6, Pages 1252-1259Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2006.05.019
Keywords
immobilization; glucoamylase; Thermomucor indicae-seudaticae; Plackett-Burman design; response surface methodology
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Alginate-entrapped sporangiospores of Thermomucor indicae-seudaticae were used for the production of glucoamylase. The critical variables that affected glucoamylase production were identified by Plackett-Burman design (sucrose, yeast-extract, K2HPO4 and asparagine) and further optimized by using a four factor central composite design (CCD) of response surface methodology (RSM). Immobilized sporangiospores secreted 41% and 60% higher glucoamylase titers in shake flasks and airlift fermenter, respectively, when the variables were used at their optimum levels (sucrose 3.0%, yeast-extract 0.2%, K2HPO4 0.1% and asparagine 0.35%). Glucoamylase production (26.3 U ml(-1)) in the optimized medium was in good agreement with the values predicted by the quadratic model (26.7 U ml(-1)), thereby confirming its validity. The enzyme production was sustainable in flasks of higher volume and also airlift fermenter, and attained a peak within 32 h in the fermenter as compared to that of 48 h in shake flasks. (c) 2006 Elsevier Ltd. All rights reserved.
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