Journal
EXPERIMENTAL HEMATOLOGY
Volume 35, Issue 4, Pages 587-595Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.exphem.2006.12.007
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Funding
- NCI NIH HHS [P01 CA108671-01A2, 1P01 CA 108671-01A2, P01 CA108671, P01 CA108671-01A20001] Funding Source: Medline
- NHLBI NIH HHS [R01 HL 50077-12, R01 HL050077-12, R01 HL050077] Funding Source: Medline
- NIDDK NIH HHS [T32 DK060445, T32 DK 60445-03] Funding Source: Medline
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Objectives. A G > T transversion in a tyrosine kinase JAK2 (V617F) was reported in over 80% of patients with polycythemia vera (PV). Current evidence suggests that JAK2(V617F) somatic mutation is involved in the pathogenesis of PV, as it confers erythropoietin-independent proliferation to erythroid progenitor cells. However, several unanswered questions regarding the essential role of JAK2(V617F) arose as 1) it is not a dominant mutation, 2) it is not PV-specitic as it is found in several myeloproliferative disorders, and 3) some (similar to 20%) PV patients lack the JAK2(V617F) mutation. We investigated the relative frequency of JAK2(V617F) in in vitro-expanded PV progenitors. Methods. In vitro expansion of erythroid progenitors from mononuclear cells was optimized. Frequency of JAK2 V617F allele was measured by using allele-specific real-time polymerase chain reaction. Clonality was performed using established procedure. Results. In vitro expansion of PV erythroid progenitors and differentiated dendritic cells resulted in a decrease of the frequency of JAK2(V617F) allele compared with granulocytes or CD235(+) erythroid progenitors. Clonality analysis demonstrated that although granulocytes of these PV patients were clonal, expanded erythroid cells were polyclonal. However, in vitro-expanded PV erythroid progenitors still had approximately a twofold increased proliferative capacity in comparison with erythroid progenitors from healthy individuals. Erythropoietin favors the cells without JAK2(V617F) allele. Dendritic cells in one out of three patients remained clonal. Conclusion. JAK2(V617F) mutation does not provide a proliferative/survival advantage to the PV clone during in vitro expansion. These data suggest that the JAK2(V617F) mutation plays an important role in the biology of PV, yet it may not be the PV-initiating event. (c) 2007 International Society for Experimental Hematology. Published by Elsevier Inc.
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