Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 29, Issue 2, Pages 157-163Publisher
SPRINGER
DOI: 10.1007/s11738-006-0020-3
Keywords
Agrobacterium-mediated transformation; beta-glucuronidase; pea; phosphinothricin
Categories
Ask authors/readers for more resources
Three methods of transformation of pea ( Pisum sativum ssp. sativum L. var. medullare) were tested. The most efficient Agrobacterium tumefaciensmediated T-DNA transfer was obtained using embryonic segments from mature pea seeds as initial explants. The transformation procedure was based on the transfer of the T-DNA region with the reporter gene uidA and selection gene bar. The expression of beta-glucuronidase (GUS) in the regenerated shoots was tested using the histochemical method and the shoots were selected on a medium containing phosphinothricin (PPT). The shoots of putative transformants were rooted and transferred to non-sterile conditions. Transient expression of the uidA gene in the tissues after co-cultivation and in the course of short-term shoot cultivation ( confirmed by histochemical analysis of GUS and by RT-PCR of mRNA) was achieved; however, we have not yet succeeded in proving stable incorporation of the transgene in the analysed plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available