Journal
JOURNAL OF PLANT DISEASES AND PROTECTION
Volume 114, Issue 2, Pages 62-68Publisher
EUGEN ULMER GMBH CO
DOI: 10.1007/BF03356205
Keywords
conidia germination; Fusarium moniliforme; 6-pentyl-alpha-pyrone; preparative HPLC; Trichoderma spp.
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The interactions between eight Trichoderma spp. isolates and Fusarium moniliforme were investigated. The results obtained from dual culture and the tests on volatile activity showed that the two isolates of T harzianum T23 and T16 exhibited considerable antagonistic potential. Quantitative analysis of 6-pentyl-alpha-pyrone (6PAP), purified from culture filtrate of T harzianum isolate T23, revealed that the optimal harvesting time for 6PAP production was 12 days post inoculation (dpi) (72.3 mg 1(-1)). In in vitro studies, 6PAP demonstrated potent inhibitory properties upon mycelial growth and sporulation of F. moniliforme in both volatile and non-volatile tests as well as on conidia germination and length of germ tubes. Mycelial growth was inhibited by 93.5% using 250 mu g ml(-1) of 6PAP. Conidia production of the target fungus was inhibited by 250 mu g ml(-1) of 6PAP in non-volatile and volatile assays by 98.0% and 78.6%, respectively. Germ tube growth was also inhibited by more than 94% using 300 mu g ml(-1) of 6PAP. The results obtained demonstrated that the metabolite 6PAP exhibited rather fungistatic than fungicidal effects.
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