Journal
JOURNAL OF FOOD PROCESS ENGINEERING
Volume 30, Issue 2, Pages 174-185Publisher
WILEY
DOI: 10.1111/j.1745-4530.2007.00102.x
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Chlorella is a promising alternative resource of lutein, as it can be cultivated heterotrophically and efficiently in a fermentor In this study, high density of Chlorella pyrenoidosa was achieved by fed-batch cultivations. Lutein in Chlorella was extracted by supercritical fluid and was determined by high-performance liquid chromatography and liquid chromatography-mass spectrometry. The extraction degree of lutein reached 87.0% after 4-h extraction under the optimized conditions of 50C, 25 MPa and modified CO2 with 50% ethanol. High purity of lutein could be obtained by supercritical fluid extraction with appropriate operation parameters. The whole process developed in this study may be useful for the commercial production of lutein.
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