The reduction of oxidative stress, anticoagulation of platelets, and inhibition of lipopolysaccharide by conjugated linoleic acid bonded on a polysulfone membrane

Conjugated linoleic acid (CLA) was covalently bonded onto the surface of a polysulfone (PSF) membrane via esterification. The surface was characterized with contact angle measurement and FT-IR spectrometery. The capability of bonded CLA on the suppression of oxidative stress was evaluated. The level of reactive oxygen species (ROS) was measured by the chemiluminescence (CL) method to evaluate the oxidative stress. Furthermore, the removal of bacterial endotoxin (lipopolysaccharide, LPS) by CLA-bonding PSF was measured with enzyme-linked immunosorbent assay (ELISA). The reduction in the platelet adhesion on the PSF surface was evaluated using a SF-3000 automated hematology analyzer. The result indicates that CLA-bonding PSF can reduce the adhesion of platelets by 73%. The concentration of LPS can be reduced by CLA-bonding PSF 1.6 times more than unmodified PSF membrane. In addition, the level of ROS against CLA-bonding PSF membrane was 23% of that against unmodified PSF membrane. Therefore CLA-bonding PSF membrane could offer protection for patients against oxidative stress and could also inhibit LPS for clinical applications. Copyright (C) 2007 John Wiley & Sons, Ltd.