Journal
VASCULAR PHARMACOLOGY
Volume 46, Issue 4, Pages 271-277Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.vph.2006.10.011
Keywords
Salvia miltiorrhiza; danshen; danshensu; calcium channel; potassium channel; coronary artery
Categories
Ask authors/readers for more resources
In this study, we have investigated the actions of danshensu, an active, water-extractable component of the medicinal herb danshen (Salvia miltiorrhiza), on rat isolated coronary artery rings precontracted with 1 mu M 5-hydroxytryptamine (5-HT) and its action compared to the water-extractable fraction of the herb. Extraction of the water-soluble fraction from danshen (S. miltiorrhiza) provided yield of 17.5% (35 g/200 g). The amount of danshensu determined in the crude danshen herb and in its aqueous fraction was 0.45 mg/g (0.045%) and 3.28 mg/g (0.33%). The danshen aqueous extract was 13 times less potent than danshensu in relaxing 5-HT-precontracted coronary artery rings; IC50 values were 930.3 +/- 133.5 mu g/mI and 71.5 +/- 11.0 mu g/ml. Removal of the endothelium did not significantly affect their vasodilator potencies; IC50 values were 842.1 +/- 123.8 mu g/ml and 84,8 +/- 8.8 mu g/ml. On the other hand, a potassium channel inhibitor tetraethylammonium (TEA, 10 mM) shifted their concentration-response curves by 1.7 and 2.2 folds. The possible involvement of Ca2+ channels was investigated in artery rings incubated with Ca2+-free buffer and primed with 1 mu M 5-HTor 60 mM KCl for 5 min priorto addition of CaCl2 to elicit contraction. In 5-HT-primed preparations, the CaCl2-induced vasoconstriction was abolished by 2 mg/mI danshen aqueous extract and 200 mu g/ml danshensu, whereas, in KCl-primed preparations, 10 mg/ml danshen aqueous extract and 600 mu g/ml danshensu were required to abrogate the vasoconstriction. These findings suggest the vasorelaxant actions of danshen aqueous extract and danshensu were produced by inhibition of Ca2+ influx in the vascular smooth muscle cells. The opening of K+ channels had a minor contribution to the response, but endothelium-dependent mechanisms were not involved. (c) 2006 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available