Journal
ULTRAMICROSCOPY
Volume 107, Issue 4-5, Pages 308-312Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2006.08.005
Keywords
SNOM; direct labelling; chromosome imaging; fluorescence in-situ hybridisation
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Scanning near-field optical microscopy (SNOM) has been successfully employed to generate high resolution (< 100 nm) fluorescence images of directly tagged human chromosomes. Direct tagging, fluorescence in-situ hybridisation processes (with and without amplification) are investigated and their fluorescence response to near-field excitation are compared. Using the simultaneous topography mode of SNOM, chromosome morphology was seen to differ as a result of the two processes; with chromatin collapse more extensive when the amplified direct tagging procedure was used. The results are discussed in the context of developing locus specific direct tags together with high resolution SNOM imaging for the observation of chromosome aberrations. (c) 2006 Elsevier B.V. All rights reserved.
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