Journal
BIOMATERIALS
Volume 28, Issue 10, Pages 1787-1797Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2006.12.020
Keywords
nanotopography; osteoblast; adhesion; integrin; focal adhesion kinase
Funding
- NIA NIH HHS [AG13087-10] Funding Source: Medline
- NIBIB NIH HHS [EB002016-13] Funding Source: Medline
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An important consideration in developing physical biomimetic cell-stimulating cues is that the in vivo extracellular milieu includes nanoscale topographic interfaces. We investigated nanoscale topography regulation of cell functions using human fetal osteoblastic (hFOB) cell culture on poly(L-lactic acid) and polystyrene (50150 w/w) demixed nanoscale pit textures (14, 29, and 45 nm deep pits). Secondary ion mass spectroscopy revealed that these nanotopographic surfaces had similar surface chemistries to that of pure PLLA because of PLLA component surface segregation during spin casting. We observed that 14 and 29 nm deep pit surfaces increased hFOB cell attachment, spreading, selective integrin subunit expression (e.g., alpha v relative to alpha 5, beta 1, or beta 3), focal adhesive paxillin protein synthesis and paxillin colocalization with cytoskeletal actin stress fibers, and focal adhesion kinase (FAK) and phosphorylated FAK (pY397) expression to a greater degree than did 45 nm deep pits or flat PLLA surfaces. Considering the important role of integrin-mediated focal adhesion and intracellular signaling in anchorage-dependent cell function, our results suggest a mechanism by which nanostructured physical signals regulate cell function. Modulation of integrin-mediated focal adhesion and related cell signaling by altering nanoscale substrate topography will have powerful applications in biomaterials science and tissue engineering. (c) 2006 Published by Elsevier Ltd.
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