Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 4, Pages 647-651Publisher
SPRINGER
DOI: 10.1007/s10529-006-9274-5
Keywords
allophycocyanin; Arthronema africanum; C-phycocyanin; method; purification; separation
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A rapid, inexpensive and reliable procedure for separation and purification of C-phycocyanin (C-PC) and allophycocyanin (APC) from Arthronema africanum based on a previously described rivanol-sulfate method for C-PC purification was developed. Exclusion of NaCl from the extraction buffer resulted in complete separation of APC and C-PC, two-fold reduction of rivanol treatments, and a higher yield and purity of C-PC. Pure C-PC (A(620)/A(280) of 4.52) and APC (A(652)/A(280) of 2.41) were obtained. The estimated molecular masses of the alpha and beta subunits were 17 and 19 kDa for C-phycocyanin and 16 and 18 kDa for APC, respectively. The overall C-PC recovery of 55% (w/w) from its content (100 mg) in the crude extract was 10-20% higher than so far reported. The procedure appears promising for scaling up and broader applications.
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