The protection effect of β-CD on DNA damage induced by ultrafine TiO2

Under the photocatelysis of 365 nm ultraviolet radiation, ultrafine TiO2 caused the oxidative damage of Teasy plasmid DNA. The damage was determined by gel-electrophoresis. Then, a different dose of O-CD was added to the reaction, and the damage was restrained. The rate of damage restraining reached 97% when the mass of P-CD was 4 times as that of TiO2. Through UV scan and IR spectroscopy, it was found that the Ti-O of ultrafine TiO2 was bound with -OH of P-CD cavurn and the -OH on the surface of ultrafine TiO2 disappeared, so the formation of center dot OH was controlled. The ultrafine TiO2 has been widely used, but it was determined to be carcinogenic by some research. The protection effect of beta-CD to DNA in the molecular level takes a new look on the surface modification of nano particles to decrease the toxic effect.