Journal
PLANT BREEDING
Volume 126, Issue 2, Pages 201-206Publisher
WILEY
DOI: 10.1111/j.1439-0523.2007.01341.x
Keywords
Nicotiana tabacum; Nicotiana glauca; ploidy level; pollen fertility; genotype background
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Nicotiana glauca is of potential interest to breeders as it carries resistance to black root rot of tobacco. Cytological investigations of sexual interspecific hybrids of N. tabacum T'T'TT (2n = 4x = 48) cv. 'Wislica' x N. glauca GG (2n = 2x = 24) were carried out. The analyses of chromosome association at diakinesis and metaphase I in the PMCs of amphihaploid F-1 T'TG (2n = 3x = 36) revealed low variable pairing with 0-5 bivalents. The sterile amphihaploids F-1 were converted into partial female fertile amphidiploids T'T'TTGG by chromosome doubling. Among 36 mature plants obtained, 15 were found to have chromosome numbers (2n = 6x = 72) and were verified as amphidiploids, 9 had (2n = 6x = 70 or 71) chromosomes while the remaining 12 were haploid. True amphidiploids, in spite of quite high chromosome pairing during meiosis, were very different in pollen fertility, ranging from 0% to 85%. Male fertility disturbances did not correlate with the degree of female fertility upon pollination with N. tabacum. Sesquidiploids T'TG (2n = 5x 60) obtained from backcrossing the amphidiploids to parental tobacco showed more than 22 bivalents, 10-12 univalents and occasional multivalents that indicated the possibility of interchange events between N. tabacum and N. glauca genomes.
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