Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altitude

Podophyllum hexandrum Royle, an important alpine herb, and a source of the highly valued aryltetralin-type lignan, podophyllotoxin, has been subjected to heavy collection from the wild due to ever increasing demand. The present study deals with an attempt to bring this plant under cultivation at a relatively lower altitude and to evaluate ( 1) various growth parameters including above and below ground biomass accumulation, net assimilation rate and relative growth rate, etc., and ( 2) podophyllotoxin content from the resulting above and below ground biomass in seed raised plants of a known age series of 1-5 years. The podophyllotoxin content was estimated on the basis of HPLC analyses. The levels were found to increase with the plant age and the maximum amount was found in 5-year old plants. This study demonstrates that ( 1) seeds can be conveniently used for raising healthy propagules in easily approachable locations at a relatively lower altitude, and that the plants can be maintained in such sites over long periods, and ( 2) this approach of conservation through cultivation'' can be suggested as an effective tool for the management of this critically endangered status'' species.