Journal
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
Volume 311, Issue 1, Pages 421-424Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmmm.2006.11.175
Keywords
GoldMag nanoparticles; biotin-avidin amplification; sandwich; immunoassay; HBsAg
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Using GoldMag (Fe3O4/Au) nanoparticles as a carrier, a biotin-avidin amplified ELISA was developed to detect hepatitis B surface antigen (HBsAg). A specific antibody was labeled with biotin and then used to detect the antigen with an antibody coated on GoldMag nanoparticles by a sandwich ELISA assay. The results showed that 5 mol of biotin were surface bound per mole of antibody. The biotin-avidin amplified ELISA assay has a higher sensitivity than that of the direct ELISA assay. There is 5-fold difference between HBsAg positive and negative serum even at dilution of 1:10000, and the relative standard deviation of the parallel positive serum at dilution of 1:4000 is 5.98% (n = 11). (C) 2006 Elsevier B.V. All rights reserved.
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