Journal
DIAMOND AND RELATED MATERIALS
Volume 16, Issue 4-7, Pages 671-674Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.diamond.2006.11.100
Keywords
diamond film; nanocrystalline; coatings; biomedical applications
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Glass slides (standard 1 x 3 in. size) coated with nanocrystalline diamond were successfully tested for DNA immobilization. The nanodiamond films were grown on glass substrates at temperature below 400 degrees C, while keeping the excellent material properties of diamond, such as low background luminescence and high optical transparency. The nanodiamond surface to which proteins were attached was functionalized by ultra-thin amino-polymer film in the radio-frequency (RF) plasma discharge of vaporized organosilane coupling agent N-(6-ammohexyl) ammopropyl trimethoxysilane (AHAPS). Several different IR spectroscopy methods (transmission and reflection-absorption spectroscopy (IRRAS), attenuated total reflectance (ATR) and grazing angle reflectance (GAR)) are discussed with respect of their ability of detecting the functional groups on bio-functionalized diamond surface. The IR absorbance spectra of just a few nm thick RF plasma polymer films deposited on nanodiamond surface are presented. (C) 2007 Published by Elsevier B.V.
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