Journal
FEBS LETTERS
Volume 581, Issue 7, Pages 1377-1382Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.02.055
Keywords
protein kinase D; PKD; proliferation; survival; NF kappa B; HSP27
Funding
- NIAID NIH HHS [AI45901, R01 AI045901] Funding Source: Medline
- Wellcome Trust [065975] Funding Source: Medline
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To investigate the importance of protein kinase D (PKD) enzymes we generated a PKD-null DT40 B-lymphocyte cell line. Previously we have shown that PKDs have an essential role in regulating class II histone deacetylases in DT40 B-cells [Matthews, S.A., Liu, P., Spitaler, M., Olson, E.N., McKinsey, T.A., Cantrell, D.A. and Scharenberg, A.M. (2006) Essential role for protein kinase D family kinases in the regulation of class 11 historic deacetylases in B lymphocytes. Mol. Cell Biol. 26, 1569-1577]. We now show that PKDs are also required to regulate HSP27 phosphorylation in DT40 B-cells. However, in contrast to previous observations in other cell types, PKD enzymes do not regulate basic cellular processes such as proliferation or survival responses, nor NF kappa B transcriptional activity downstream of the B cell antigen receptor. Thus, PKDs have a selective role in DT40 B-cell biology. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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