Journal
SYNLETT
Volume -, Issue 6, Pages 870-873Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/s-2007-973869
Keywords
oligonucleotides; fluorescence detection; 2 '-deoxy-pyrrolocytidine; single nucleotide polymorphism analysis
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The intrinsically fluorescent nucleoside 2'-deoxy-6-phenylpyrrolocytidine when incorporated into an oligonucleotide, is selectively quenched by hybridization with match DNA vs. mismatched sequences.
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