4.4 Article

Selective fluorometric detection of guanosine-containing sequences by 6-phenylpyrrolocytidine in DNA

Journal

SYNLETT
Volume -, Issue 6, Pages 870-873

Publisher

GEORG THIEME VERLAG KG
DOI: 10.1055/s-2007-973869

Keywords

oligonucleotides; fluorescence detection; 2 '-deoxy-pyrrolocytidine; single nucleotide polymorphism analysis

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The intrinsically fluorescent nucleoside 2'-deoxy-6-phenylpyrrolocytidine when incorporated into an oligonucleotide, is selectively quenched by hybridization with match DNA vs. mismatched sequences.

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