Journal
FEBS LETTERS
Volume 581, Issue 7, Pages 1302-1310Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.02.042
Keywords
cytochrome c oxidase; subunit phosphorylation; myocardial ischemia/reperfusion; protein kinase A; nano-LC-MS/MS analysis
Funding
- NIGMS NIH HHS [GM-49683, R01 GM049683] Funding Source: Medline
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We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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