Journal
FEBS LETTERS
Volume 581, Issue 7, Pages 1505-1508Publisher
WILEY
DOI: 10.1016/j.febslet.2007.03.006
Keywords
iron metabolism; Redox titration; trans-Membrane electron transfer; two-heme cytochrome b561; vacuole
Funding
- NCRR NIH HHS [1P20RR17675] Funding Source: Medline
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Ascorbate-reducible cytochromes b561 (Cyts-b561) are a class of intrinsic trans-membrane proteins. Tonoplast Cyt-b561 (TCytb), one of the four Cyt-b561 isoforms in Arabidopsis was localized to the tonoplast. We demonstrate here that the optical spectra, EPR spectra and redox potentials of recombinant TCytb are similar to those of the well characterized bovine chromaffin granule Cyt-b561. We provide evidence for the reduction of ferric-chelates by the reduced TCytb. It is also shown that TCytb is capable of trans-membrane electron transport from intracellular ascorbate to extracellular ferric-chelates in yeast cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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