Enantioseparation of some chiral flavanones using microbial cyclic β-(1→3),(1→6)-glucans as novel chiral additives in capillary electrophoresis

Cyclic beta-(1 -> 3),(1 -> 6)-glucans, microbial cyclooligosaccharides produced by Bradyrhizobium japonicum USDA 110, were used as novel chiral additives for the enantiomeric separation of some flavanones such as eriodictyol, homoeriodictyol, hesperetin, naringenin, and isosakuranetin in capillary electrophoresis (CE). Among the flavanones, eriodictyol was separated with the highest resolution (R-s 5.66) and selectivity factor (alpha 1.18) when 20 mM cyclic beta-(1 -> 3),(1 -> 6)-glucans were added to the background electrolyte (BGE) at pH 8.3. (c) 2007 Elsevier Ltd. All rights reserved.