Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 115, Issue 2, Pages 187-194Publisher
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2006.10.022
Keywords
Listeria monocytogenes; food-borne pathogen; MLVA; subtyping Listeria monocytogenes
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In this study we report the development and application of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) strategy for subtyping Listeria monocytogenes. Genome profiles of a collection of forty-five food-borne L. monocytogenes isolates were compared using MLVA. These isolates were obtained as part of an active surveillance programme of foods in the south-east region of Ireland. MLVA successfully discriminated amongst the isolates. The method was easy to perform, relatively fast and could be deployed in any molecular laboratory with basic laboratory equipment. This approach is a valuable tool, which has the capability to provide comparable results when compared with other more established typing methods, including pulsed-field gel electrophoresis (PFGE). (c) 2006 Elsevier B.V. All rights reserved.
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