Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 849, Issue 1-2, Pages 43-52Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2006.10.069
Keywords
cancer; proteomics; plasma proteome; serum proteome; blood proteome; multidimensional separation; biomarkers; protein separation; peptide separation
Funding
- NCI NIH HHS [CA77048, CA10815] Funding Source: Medline
- BHP HRSA HHS [SA4100020718] Funding Source: Medline
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The plasma proteome has a wide dynamic range of protein concentrations and is dominated by a few highly abundant proteins. Discovery of novel cancer biomarkers using proteomics is particularly challenging because specific biomarkers are expected to be low abundance proteins with normal blood concentrations of low nanograms per milliliter or less. Conventional, one- and two-dimensional proteomic methods including 2D PAGE, 2D DIGE, LC-MS/MS, and LC/LC-MS/MS do not have the capacity to consistently detect many proteins in this range. In contrast, new higher dimensional (Hi-D) separation strategies, utilizing more than two dimensions of fractionation, can profile the low abundance proteome. (c) 2006 Elsevier B.V. All rights reserved.
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