Journal
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Volume 10, Issue 2, Pages 328-335Publisher
UNIV CATOLICA DE VALPARAISO
DOI: 10.2225/vol10-issue2-fulltext-2
Keywords
differentiation; EB; ES; murine embryonic stem cell
Categories
Ask authors/readers for more resources
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES such as is performed in high-throughput screening protocols.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available