Journal
ANALYTICAL BIOCHEMISTRY
Volume 363, Issue 2, Pages 239-245Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2007.01.030
Keywords
fluorescence immunoassay; metal-enhanced fluorescence; plasmonics; nano-size metal particles; silver island films
Funding
- NCI NIH HHS [CA 114460] Funding Source: Medline
- NCRR NIH HHS [P41 RR008119] Funding Source: Medline
- NIBIB NIH HHS [R33 EB001690, EB-1690] Funding Source: Medline
- PHS HHS [NCRR-08119] Funding Source: Medline
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We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement. (c) 2007 Elsevier Inc. All rights reserved.
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