Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 204, Issue 4, Pages 819-830Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20062104
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Funding
- Intramural NIH HHS Funding Source: Medline
- NIAID NIH HHS [R01AI50659, R01AI43576, R01 AI043576, R01 AI050659] Funding Source: Medline
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Memory B cells provide rapid protection to previously encountered antigens; however, how these cells develop from germinal center B cells is not well understood. A previously described in vitro culture system using human tonsillar germinal center B cells was used to study the transcriptional changes that occur during differentiation of human memory B cells. Kinetic studies monitoring the expression levels of several known late B cell transcription factors revealed that BCL-6 is not expressed in memory B cells generated in vitro, and gene expression profiling studies confirmed that BCL-6 is not expressed in these memory B cells. Furthermore, ectopic expression of BCL-6 in human B cell cultures resulted in formation of fewer memory B cells. In addition, the expression profile of in vitro memory B cells showed a unique pattern that includes expression of genes encoding multiple costimulatory molecules and cytokine receptors, antiapoptotic proteins, T cell chemokines, and transcription factors. These studies establish new molecular criteria for defining the memory B cell stage in human B cells.
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