Journal
FEBS LETTERS
Volume 581, Issue 8, Pages 1561-1566Publisher
WILEY
DOI: 10.1016/j.febslet.2007.03.013
Keywords
trans-2-enoyl-CoA reductase; NADH-dependent; crotonoyl-CoA; fatty acid biosynthesis; Treponema denticola
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An NADH-dependent trans-2-enoyl-CoA reductase (EC1.1.1.36) from the Gram negative spirochete Treponema denticola was identified, expressed and biochemically characterized. The recombinant protein is a monomeric enzyme with a molecular mass of 44 kDa with a specific activity of 43 +/- 4.8 U/mg (mu mol mg(-1) min(-1)) and K-m value of 2.7 mu M for crotonoyl-CoA. This NADH-dependent trans-2-enoyl-CoA reductase represents the first enzymatically characterized member of a prokaryotic protein family involved in a fatty acid synthesis pathway that is distinct from the familiar fatty acid synthase system. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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