Journal
JOURNAL OF NEUROSCIENCE
Volume 27, Issue 16, Pages 4297-4302Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0399-07.2007
Keywords
radial glia; subventricular zone; olfactory bulb; interneuron; dorsal; development; postnatal
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Funding
- NINDS NIH HHS [F30 NS051997, R01 NS017125, F30 NS-051997, NS-017125, F30 NS051997-02] Funding Source: Medline
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During embryogenesis, dorsal radial glia generate pyramidal cell neurons but not interneurons, and are thought to degenerate or transform into astrocytes in the postnatal brain. Ventral radial glia, in contrast, retract their processes to form GFAP + subventricular zone (SVZ) astrocytes that continue to generate interneurons into adulthood. We sought to fate map dorsal radial glia by codelivering an adenovirus expressing Cre recombinase and a lentivirus expressing dsRedExpress to the dorsal cortical surface of ROSA26R-YFP mice. Whereas the adenovirus is retrogradely transported to the cell body, the VSV-G ( vesicular stomatitis virus G) pseudotyped lentivirus is not and allows us to control for viral diffusion from the site of infection. We found that dorsal radial glia give rise to gray and white matter astrocytes and oligodendrocytes. In addition, the dorsal radial glia fate map to astrocytes lining the dorsal aspect of the SVZ that persist at least 8 weeks postnatally. Finally, we found that dorsal radial glial-derived cells generate granule cell and periglomerular interneurons in the olfactory bulb and continue to form interneuronal precursors into adulthood.
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