Journal
JOURNAL OF ETHNOPHARMACOLOGY
Volume 111, Issue 1, Pages 1-7Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2006.09.046
Keywords
Plectranthus barbatus; Lamiaceae; acid secretion; rabbit gastric glands; H+,K+-ATPase; plectrinone A
Ask authors/readers for more resources
This work assessed the mechanism underlying the antisecretory gastric acid effect of Plectranthus barbatus Andrews (Lamiaceae) and active constituents. Popularly known as false-boldo, this plant is used in Brazilian folk medicine to treat gastrointestinal and hepatic ailments. The plant aqueous extract (AE) and isolated compounds were assayed in vivo in pylorus-ligated mice, and in vitro on acid secretion measured as [C-14] -arninopyrine ([C-14]-AP) accumulation in rabbit gastric glands and gastric H+,K+-ATPase preparations. Injected into the duodenal lumen, the AE of the plant leaves (0.5 and 1.0g/kg) decreased the volume (62 and 76%) and total acidity (23 and 50%) of gastric acid secretion in pylorus-ligated mice. Bioguided purification of the AE yielded an active fraction (IC50 = 24 kg/ml) that inhibited acid secretion in rabbit gastric glands with a potency 10 to 18 times greater than that of the originating extract, on both the basal and stimulated acid secretion by histamine (His) (1 mu M) or bethanechol (100 mu M). At the same concentrations the gastric H+,K+-ATPase activity was also inhibited. The active constituent was chemically identified as the abietanoid dienedione plectrinone A which reduced the H+,(+)-ATPase activity with IC50 = 171 mu M. The results indicate that inhibition of the gastric proton pump by this diterpenoid may account for the antisecretory acid effect and reputed antiulcer activity of Plectranthus barbatus. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available