Journal
JOURNAL OF CELL BIOLOGY
Volume 177, Issue 2, Pages 243-252Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200609038
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Funding
- NCRR NIH HHS [P41 RR001081, P41 RR-01081] Funding Source: Medline
- NIGMS NIH HHS [GM073847, R01 GM073847] Funding Source: Medline
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The outer dynein arms (ODAs) of the flagellar axoneme generate forces needed for flagellar beating. Elucidation of the mechanisms underlying the chemomechanical energy conversion by the dynein arms and their orchestrated movement in cilia/flagella is of great importance, but the nucleotide-dependent three-dimensional (3D) movement of dynein has not yet been observed. In this study, we establish a new method for reconstructing the 3D structure of the in vitro reconstituted ODA-microtubule complex and visualize nucleotide-dependent conformational changes using cryoelectron microscopy and image analysis. As the complex went from the rigor state to the relaxed state, the head domain of the beta heavy chain shifted by 3.7 nm toward the B tubule and inclined 44 degrees inwards. These observations suggest that there is a mechanism that converts head movement into the axonemal sliding motion.
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