Journal
JOURNAL OF CONTROLLED RELEASE
Volume 118, Issue 3, Pages 294-302Publisher
ELSEVIER
DOI: 10.1016/j.jconrel.2006.12.023
Keywords
peptide; M cells; in vitro model of the human follicle-associated epithelium (FAE); polymeric nanoparticles; oral delivery; helodermin
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M cells represent a potential portal for oral delivery of peptides and proteins due to their high endocytosis abilities. An in vitro model of human FAE (co-cultures) was used to evaluate the influence of M cells on the transport of free and encapsulated helodermin - a model peptide - across the intestinal epithelium. M cells enhanced transport of intact helodermin (18-fold, Papp 3 X 10(-6) cm s(-1)). As pegylation increased nanoparticle transport by M cells, helodermin was encapsulated in 200 mu nanoparticles containing PEG-b-PLA:PLGA 1:1. Stability of the selected formulation was demonstrated in simulated gastric and intestinal fluids. M cells increased the transport of helodermin encapsulated in these nanoparticles by a factor of 415, as compared to Caco-2 cells. Transport of free and encapsulated helodermin occurred most probably by endocytosis. In conclusion, M cells improved helodermin transport across the intestinal epithelium, confirming their high potential for oral delivery of peptides. (c) 2007 Elsevier B.V. All rights reserved.
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