Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 17, Pages 7080-7085Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0702409104
Keywords
bone marrow transplantation; hematopoiesis; hematopoietic stem/progenitor cell; lymphopoiesis
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Funding
- NCI NIH HHS [P30 CA008748] Funding Source: Medline
- NIDDK NIH HHS [R01 DK068348, R01 DK 068343] Funding Source: Medline
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MicroRNAs (miRNAs) are a family of approximate to 22-nt noncoding RNAs that can posttranscriptionally regulate gene expression. Several miRNAs are specifically expressed in hematopoietic cells. Here we show that one such miRNA, miR-150, is mainly expressed in the lymph nodes and spleen and is highly up-regulated during the development of mature T and IS cells; expression of miR-150 is sharply up-regulated at the immature B cell stage. Overexpression of miR-150 in hernatopoietic stem cells, followed by bone marrow transplantation, had little effect on the formation of either mature CD8- and CD4-positive T cells or granulocytes or macrophages, but the formation of mature B cells was greatly impaired. Furthermore, premature expression of miR-150 blocked the transition from the pro-B to the pre-B stage. Our results indicate that miR-150 most likely down-regulates mRNAs that are important for pre- and pro-B cell formation or function, and its ectopic expression in these cells blocks further development of B cells.
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