Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 17, Pages 7039-7044Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0702137104
Keywords
mechanical force; misfolding; optical tweezers; RNA folding; single molecule
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Funding
- NIGMS NIH HHS [GM 32543, R37 GM032543, R01 GM010840-48, R01 GM010840-49, R01 GM010840, R01 GM032543, R37 GM010840, GM 10840] Funding Source: Medline
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The rugged folding-energy landscapes of RNAs often display many competing minima. How do RNAs discriminate among competing conformations in their search for the native state? By using optical tweezers, we show that the folding-energy landscape can be manipulated to control the fate of an RNA: individual RNA molecules can be induced into either native or misfolding pathways by modulating the relaxation rate of applied force and even be redirected during the folding process to switch from misfolding to native folding pathways. Controlling folding pathways at the single-molecule level provides a way to survey the manifold of folding trajectories and intermediates, a capability that previously was available only to theoretical studies.
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