Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 17, Pages 7175-7180Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0610442104
Keywords
T cell activation; regulation of transcription
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Funding
- NCRR NIH HHS [P20 RR021905] Funding Source: Medline
- NIAID NIH HHS [P02 AI 045666, P01 AI045666] Funding Source: Medline
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In contrast to naive CD4(+) T cells, memory CD4(+) T cells rapidly express high levels of effector cytokines in response to antigen stimulation. The molecular mechanism for this specific behavior is not well understood. The nuclear factor of activated T cells (NFAT) family of transcription factors plays an important role in the transcription of many cytokine genes. Here we show that memory CD4(+) T cells rapidly induce NFAT-mediated transcription upon T cell receptor ligation whereas NFAT activation in naive CD4(+) T cells requires longer periods of stimulation. The difference in kinetics correlates with the low levels of NFATc1 and NFATc2 proteins present in naive CD4(+) T cells and their high levels in memory CD4(+) T cells. Accordingly, IL-2 expression requires NFAT activation only in memory CD4(+) T cells whereas it is NFAT-independent in naive CD4(+) T cells. Thus, the accumulation of NFATc1 and NFATc2 in memory CD4(+) T cells represents a previously uncharacterized regulatory mechanism for the induction of early gene expression after antigen stimulation.
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