Journal
EXPERIMENTAL AND MOLECULAR MEDICINE
Volume 39, Issue 2, Pages 239-245Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/emm.2007.27
Keywords
CpG-oligonucleotide; extracellular signal-regulated MAP kinases; matrix metalloproteinase 9; NF-kappa B; p38 mitogen-activated protein kinases; Toll-like receptor 9
Ask authors/readers for more resources
Unmethylated CpG oligodeoxynucleotides (CpG ODNs) activate immune cells to produce immune mediators. This study demonstrates that in murine macrophage RAW 264.7 cells, CpG ODN-mediated matrix metalloproteinase-9 (MMP-9) expression is regulated at transcriptional level and requires de novo protein synthesis. Inhibition of ERK and p38 MAPK, but not JNK, results in significant decrease of CpG ODN-induced MMP-9 expression. We found that endosomal maturation inhibitors, chloroquine and bafilomycin A, block CpG ODN-induced ERK and p38 MAPK activation and the subsequent MMP-9 expression. We also observed that CpG ODN induces NF-kappa B activation and NF-kappa B is a downstream target of p38 MAPK. Taken together, our data demonstrate that CpG ODN triggers MMP-9 expression via TLR-9 dependent ERK and p38 MAPK activation followed by NF-kappa B activation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available