Journal
OPTICS EXPRESS
Volume 15, Issue 9, Pages 5366-5375Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.15.005366
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This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging. (C) 2007 Optical Society of America.
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