Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 322, Issue 1-2, Pages 12-19Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2007.01.026
Keywords
biosensors; electrochemical sensors; immunosensor; immunoassay; carcinoma antigen-125; carbon nanofiber
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A carbon nanomaterial, soluble carbon nanofiber, was used for the first time to construct an immunosensor for a rapid separation-free immunoassay. The acidic oxidation of the carbon nanofiber provided its solubility and wettability for convenient preparation of a porous carbon nanofiber membrane and a larger number of active sites for covalent binding of carcinoma antigen-125 (CA125) and thionine as electron transfer mediator. This matrix was a suitable environment for the immobilized protein. The immobilized HRP-labeled immunoconjugate showed good enzymatic activity for the oxidation of thionine by hydrogen peroxide. With a competitive mechanism, the differential pulse voltammetric peak current of this system decreased linearly with increasing CA125 concentration (from 2 to 75 U/ml) in the incubation solution. The CA125 immunosensor showed good precision, high sensitivity, acceptable stability and reproducibility with a detection limit of 1.8 U/ml. The soluble carbon nanofiber is a novel method for preparation of immunosensors. (C) 2007 Elsevier B.V. All rights reserved.
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