Journal
PLANT CELL REPORTS
Volume 26, Issue 5, Pages 611-615Publisher
SPRINGER
DOI: 10.1007/s00299-006-0262-y
Keywords
alfalfa; senescence; SAG12-IPT
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We report the successfull delay of leaf senescence in Medicago sativa. A highly regenerable clone of alfalfa was transformed with the construct SAG12-IPT, an approach that has already proved efficient in other crops. Several independent transformants were obtained as determined by Southern analysis and all the transformants expressed the transgene as measured by RT-PCR. In vitro and in vivo analyses showed that SAG12-IPT plants exhibited a stay-green phenotype that has the potential to greatly improve the quantity and quality of alfalfa forage.
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