Journal
FEBS LETTERS
Volume 581, Issue 9, Pages 1891-1897Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.03.076
Keywords
DNA transfection; gene delivery; green fluorescent protein; intracellular trafficking; protein transduction domain; transgenic plants
Ask authors/readers for more resources
The delivery and expression of exogenous genes in plant cells have been of particular interest for plant research and biotechnology. Here, we present results demonstrating a simple DNA transfection system in plants. Short arginine-rich intracellular delivery peptide, a protein transduction domain, was capable of delivering plasmid DNA into living plant cells non-covalently. This peptide-mediated DNA delivery conferred several advantages, such as nuclear targeting, non-toxic effect, and ease of preparation without protoplast formulation. Thus, this novel technology shall provide a powerful tool to investigate gene function in vivo, and lay the foundation for the production of transgenic plants in future. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available