Journal
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
Volume 292, Issue 5, Pages G1411-G1419Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpgi.00557.2006
Keywords
immature intestinal epithelium; inflammation; development; necrotizing enterocolitis
Categories
Funding
- NICHD NIH HHS [K08 HD-043839] Funding Source: Medline
- NIDDK NIH HHS [K01 DK-075386, K08 DK-064840, DK-42086] Funding Source: Medline
Ask authors/readers for more resources
Premature infants are susceptible to many conditions that are inflammatory in nature. For this patient population, which is expecting the intrauterine environment, pathways necessary for fetal life and development may not have completed the transitions necessary for extrauterine life. In this study, responses to tumor necrosis factor-alpha were compared in human fetal and adult intestinal epithelial cell lines along with preweaned and postweaned mouse intestinal sections to identify a potential developmental difference that may explain the heightened inflammatory response of preterm infants. The nuclear factor-kappa B ( NF-kappa B) pathway regulates a wide variety of genes involved in immune and inflammatory processes. We report that, compared with adult intestinal epithelial cells, immature intestinal epithelial cells have increased NF-kappa B activity associated with increased NF-kappa B- DNA binding and transcriptional activity. This increased activity appears due to inadequate inhibition of signaling leading to NF-kappa B activation since there is also increased phosphorylation, ubiquitination, and degradation of the inhibitor of NF-kappa B in conjunction with decreased baseline expression and delayed resynthesis of this inhibitor. Thus we demonstrate a potential mechanism for the heightened inflammatory response of immature intestinal epithelial cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available