Journal
BIOELECTROCHEMISTRY
Volume 70, Issue 2, Pages 250-256Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2006.09.009
Keywords
biosensors; glucose oxidase; hexagonal mesoporous silica; chemically modified electrode; direct electrochemistry; glucose
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The direct electrochemistry of glucose oxidase (GOD) immobilized on a hexagonal mesoporous silica modified glassy carbon electrode was investigated. The adsorbed GOD displayed a pair of redox peaks with a formal potential of -417 mV in 0.1 M pH 6.1 phosphate buffer solution (PBS). The response showed a diffusion-controlled electrode process with a two-electron transfer coupled with a two-proton transfer reaction process. GOD immobilized on a hexagonal mesoporous silica retained its bioactivity and stability. In addition, the immobilized GOD could electrocatalyze the oxidation of glucose to gluconlactone by taking ferrocene monocarboxylic acid (FMCA) as a mediator in N-2 saturated solutions, indicating that the electrode may have the potential application in biosensors to analyze glucose. The sensor could exclude the interference of commonly coexisted uric acid, p-acetaminophenol and ascorbic acid and diagnose diabetes very fast and sensitively. This work demonstrated that the mesoporous silica provided a novel matrix for protein immobilization and the construction of biosensors. (C) 2006 Elsevier BN. All rights reserved.
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