Journal
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
Volume 40, Issue 3, Pages 264-267Publisher
SOC BRASILEIRA MEDICINA TROPICAL
DOI: 10.1590/S0037-86822007000300002
Keywords
Copaifera reticulata; Aedes aegypti; terpenoids; larvicidal activity
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Oil-resin fractions from Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) were evaluated for larvicidal activity on third larval instars of Aedes aegypti, in searching for alternative control methods for this mosquito. The bioactive fractions were chemically monitored by thin layer chromatography, H-1 and C-13 nuclear magnetic resonance and mass spectrometry. Bioassays were performed using five repetitions, at a temperature of 28 +/- 1 degrees C, relative humidity of 80 +/- 5% and light and dark cycles of 12h. Mortality was indicated by darkening of the cephalic capsule after 24h of exposure of the larvae to the solutions. The most active fractions were CRM1-4 (sesquiterpenes) and CRM5-7 (labdane diterpenes), which showed LC50 values of 0.2 and 0.8ppm, respectively
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