Journal
INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH
Volume 77, Issue 3, Pages 158-165Publisher
VERLAG HANS HUBER
DOI: 10.1024/0300-9831.77.3.158
Keywords
iron bioavailability; in vitro methods; Caco-2 cells
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An update on in vitro methods employed to assess iron bioavailability is presented. Solubility and dialyzability are not useful predictors of iron absorption, whereas Caco-2 cell models provide useful comparisons, on the availability of iron from different sources. Strengths and weaknesses of in vitro approaches are briefly described, including the need to characterize Caco-2 cells in order to interpret results correctly. Further developments are required to refine Caco-2 model systems, including optimization and standardization of methods.
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