Journal
LEUKEMIA RESEARCH
Volume 31, Issue 5, Pages 643-651Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2006.08.006
Keywords
nanotechnology; cell tracking; hematopoiesis; fluorescence microscopy
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Funding
- NCI NIH HHS [R01 CA 26038-22, R01 CA026038] Funding Source: Medline
- NHLBI NIH HHS [R01 HL 077912, P01 HL 073104, R01 HL077912, P01 HL073104, 5T32 HL 66992 01 04] Funding Source: Medline
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Quantum dots (QDs) are nanometer scale fluorescent semiconductors that are increasingly used as labeling tools in biological research. These nanoparticles have physical properties, such as high quantum yield and resistance to photobleaching, that make them attractive molecular probes for tracking hematologic cells. Here, we show that QDs attached to a transporter protein effectively label all hematologic cells tested, including cell lines and malignant and non-malignant patient samples. We demonstrate that dividing cells can be tracked through at least four cell divisions. In leukemic cell lines, some cells remain labeled for 2 weeks. We show that QDs can be used to follow cells as they differentiate. QDs are seen in monocyte-like and neutrophil-like progeny of labeled HL-60 myeloblasts exposed to Vitamin D analogues and DMSO, respectively. QDs are also observed in monocytes generated from labeled CD34+ cells. In addition, QDs attached to streptavidin can target cells with differing cell surface markers, including CD33. In summary, QDs have the ability to bind to specific cells of interest, be taken up by a diverse range of hematologic cells, and followed through many divisions and through differentiation. These results establish QDs as extremely useful molecular imaging tools for the study of hematologic cells. (c) 2006 Elsevier Ltd. All rights reserved.
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