Journal
JOURNAL OF SEPARATION SCIENCE
Volume 30, Issue 7, Pages 1005-1012Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200600397
Keywords
capillary-HPLC; monolithic column; polypeptides; proteins
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In this paper, a poly(styrene-octadecene-divinylbenzene) (PS-OD-DVB) monolithic column was prepared in one step by introducing a C-18 carbon chain as monomer. N,N-Dimethylformamide and decanol served as porogens to make a homogeneous polymerization mixture in a fused silica capillary (320 mu m inner diameter). Its physical and chromatographic properties were compared with those of poly(styrene-divinylbenzene) (PS-DVB) monolithic column, which was also fabricated by in-situ polymerization in a fused silica capillary with the same inner diameter. Six standard proteins were used to evaluate the columns and their potential application for the separation of human hemoglobin was also discussed. It was shown that the PS-OD-DVB and PS-DVB monoliths appeared to have similar efficiency for rapid separation of six proteins within 3.5 min. The PS-OD-DVB monolith was found to have higher loading capacity and higher resolution for the separation of alpha and beta chains of hemoglobin because of the introduction of C-18 carbon chains, and shows great potential for the separation of bio-macromolecules.
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