Journal
MICROBES AND INFECTION
Volume 9, Issue 6, Pages 729-734Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.micinf.2007.02.016
Keywords
Francisella tularensis; Galleria mellonella; Tularemia; phagocytosis
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Funding
- NIAID NIH HHS [K08 AI063084-02, K08 AI063084-03, U54 AI057159, AI057159, AI63084, K08 AI063084-01, K08 AI063084] Funding Source: Medline
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We used the killing of Galleria mellonella (Lepidoptera: Pyralidae; the greater wax moth) caterpillar by the live vaccine strain (LVS) of Francisella tularensis to develop an invertebrate host system that can be used to study F. tularensis infection and the in vivo effects of antibacterial compounds on F. tularensis LVS. After injection into the insect hemocoel, F. tularensis LVS, killed caterpillars despite the association of LVS with hemocytes. The rate of killing depended on the number of bacteria injected. Antibiotic therapy with ciprofloxacin, levofloxacin or streptomycin administered before or after inoculation prolonged survival and decreased the tissue burden of F. tularensis in the hemocoel. Delayed drug treatment reduced the efficacy of antibacterials and especially streptomycin. The G. mellonella-F. tularensis LVS system may facilitate the in vivo study of F. tularensis, efficacy with antibacterial agents. (C) 2007 Elsevier Masson SAS. All rights reserved.
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