Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 5, Pages 819-822Publisher
SPRINGER
DOI: 10.1007/s10529-007-9309-6
Keywords
carboxylation; gamma-resorcylic acid; resorcinol; reversible decarboxylase; Rhizobium radiobacter
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A recombinant Escherichia coli, expressing the rdc gene, which encodes a gamma-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to gamma-resorcylic acid with a 44% (mol/mol) yield at 30 degrees C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.
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