Journal
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Volume 292, Issue 5, Pages C1690-C1700Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00384.2006
Keywords
human; cell activation
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Funding
- NIDDK NIH HHS [DK-62389] Funding Source: Medline
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A comprehensive analysis of the role of the actin cytoskeleton in exocytosis of the four different neutrophil granule subsets had not been performed previously. Immunoblot analysis showed that, compared with plasma membrane, there was less actin associated with secretory vesicles ( SV, 75%), gelatinase granules ( GG, 40%), specific granules ( SG, 10%), and azurophil granules ( AG, 5%). Exocytosis of SV, SG, and AG was measured as increased plasma membrane expression of CD35, CD66b, and CD63, respectively, with flow cytometry, and GG exocytosis was measured as gelatinase release with an ELISA. N- formylmethionyl- leucyl- phenylalanine ( FMLP) stimulated exocytosis of SV, GG, and SG with an ED50 of 15, 31, and 28 nM, respectively, with maximal response at 10(-7) M FMLP by 5 min, while no exocytosis of AG was detected. Disruption of the actin cytoskeleton by latrunculin A and cytochalasin D induced a decrease in FMLP- stimulated CD35 expression after an initial increase. Both drugs enhanced the rate and extent of FMLP-stimulated GG, SG, and AG exocytosis, while the EC50 for FMLP was not altered. We conclude that the actin cytoskeleton controls access of neutrophil granules to the plasma membrane, thereby limiting the rate and extent of exocytosis of all granule subsets. Differential association of actin with the four granule subsets was not associated with graded exocytosis.
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